We present a video content analysis open source utilizing electronic fluorescence lifetime imaging (FLIM) for testing protein associations using Förster resonation essentialness trade (FRET) based readouts of fixed or live cells in multiwell plates. This gives an approach to screen to cell hailing systems read out using intramolecular FRET biosensors or intermolecular FRET of protein joint efforts, for instance, oligomerization or heterodimerization, which can be used to perceive confining assistants. We depict here the handiness of this motorized multiwell plate FLIM instrumentation and present model data from our examinations of HIV Gag protein oligomerization and a period course of a FRET biosensor in live cells.